DISC ONE
Episodes (with Play All) (178:49)
- "Dia De Los Dangerous!" (22:10)
- "Careers In Science" (21:35)
- "Mid-life Chrysalis" (22:09)
- "Eeney, Meeney, Miney… Magic!" (22:35)
- "The Incredible Mr. Brisby" (22:27)
- "Tag Sale – You're It!" (22:29)
- "Home Insecurity" (22:21)
- "Ghosts of the Sargasso" (23:00)
Audio Commentary on "Mid-life Chrysalis" with Jackson Publick and Doc Hammer
Audio Commentary on "Eeney, Meeney, Miney… Magic!" with Jackson Publick and Doc Hammer
Audio Commentary on "Tag Sale – You're It!" with Jackson Publick and Doc Hammer
Audio Commentary on "Ghosts of the Sargasso" with Jackson Publick and Doc Hammer

DISC TWO
Episodes (with Play All) (113:48)
- "Ice Station – Impossible!" (22:36)
- "Are You There, God? It's Me, Dean" (22:33)
- "Past Tense" (22:33)
- "The Trial of the Monarch" (22:28)
- "Return to Spider-Skull Island" (23:36)
Audio Commentary on "Return to Spider-Skull Island" with Jackson Publick and Doc Hammer
"The Terrible Secret of Turtle Bay" [2003 Pilot Episode] (25:04)
- Audio Commentary with Jackson Publick and Doc Hammer
"A Very Venture Christmas" [Special] (11:26)
Deleted Scenes
- "Dia De Los Dangerous!" (0:34)
- "The Incredible Mr. Brisby" (1:07)
- "Are You There, God? It's Me, Dean" (1:17)
- "Past Tense" (1:44)
- "The Trial of the Monarch" (0:52)
- "Return to Spider-Skull Island" (0:44)
"Behind the Scenes of the Live-Action Movie" featurette (21:23)
"Animating Hank and Dean" featurette (4:28)

English, French, and Spanish (Optional)

1.33:1

Non-Anamorphic

NTSC

English Dolby Digital 2.0 Stereo

Digipak